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- Director Zack Snyder was responsible for directing the specific scene where Flash appears at the beginning of the film. The scene was filmed during principal photography for the film Justice League. Coincidentally, stock footage from the previous film he directed, Batman v Superman: Dawn of Justice was also included in the intro, showing the funeral of Superman.
- Keeping the theme from the comic continuity of the Suicide Squad members being expendable assets, Slipknot and El Diablo die.
- Killer Croc is not normally part of the Suicide Squad in the comics.
- He was added to the team roster for the film because it was felt that a CGI character like King Shark wouldn't blend into the realistic setting of the film.
- Captain Boomerang tricking Slipknot into testing whether or not the bombs were real was taken straight from the first Suicide Squad story.
- Ironically enough, Slipknot is not given a file in Waller's introduction of potential Squad members.
- Although, in the comic story, the bombs only blew off the victim's arms.
- The flashback to how Dr. Quinzel becomes Harley Quinn is slightly different from the comics. In the comics, Joker pushed her into the vat, while in the film, Harleen falls willingly.
- How Joker managed to escape the crashing/burning helicopter is never revealed.
- This situation appears to have been adapted from the animated film Batman: Assault on Arkham, where the Joker is trapped in a falling helicopter by Deadshot and left for dead.
- Harley's bomb was disabled when Joker attempted to rescue her, yet she was intimidated by Waller's threats to detonate it. It's possible she re-activated the bomb, or at the moment, Harley forgot.
- The moment of Deadshot helping Harley after she fell is a nod to their short-lived romance in the comics.
- Perks the Squad get back at Belle Reve.
- Harley gets a bed and espresso machine. She also appears to be reading a provocative book.
- Deadshot is allowed supervised visits to his daughter and gets her letters.
- Croc is given the BET network to watch with better home comforts.
- Batman was responsible for Deadshot, Harley Quinn and Killer Croc being drafted into the Suicide Squad due to either arresting them or chasing them out of Gotham City.
- Katana speaks very little in the film, except for her flashback, greeting Flag for the mission and when preparing to face Enchantress. Many fans have misunderstood this for Katana not being fluent in English.
- Lobo Web series villain Sunny Jim's face appeared in the front of a store in the film.
- In the film, there is a slight goof which occurs around the 54:21-32 mark where Harley Quinn's actress Margot Robbie briefly says her lines in her natural Australian accent while talking to Deadshot before switching back to her character's American accent.